Comparitive Evaluation of Various Staining Techniques for Identification of Tubercle Bacilli by Direct Microscopy
نویسنده
چکیده
The aim of this study was to compare Fluorescent(auraminO rhodamine,AO)method ,Ziehl-Neelsen (ZN hot method)and kinyoun(cold method) staining techniques for the diagnosis of Mycobacterium tuberculosis. Materials And Methods: 186(on 3 consecutive days) sputum samples were collected from 62 suspected pulmonary tuberculosis patients, processed by petroffs method, and subjected to direct microscopy by three methods Fluorescent (auramin O rhodamine) method ,ZiehlNeelsen (ZN hot method)and kinyoun (cold method) staining techniques for the diagnosis of tuberculosis. Results: out of 62 patients 30.35%,17.8%and 17.8% were found positive,by AO,ZN and Kinyouns methods respectively. AO was found to be superior to ZN and Kinyoun methods in several aspects. AO was also able to detect more pauci-bacillary cases than ZN. Conclusion: Direct Microscopy is still the most simple and reliable method for diagnosis of Tuberculosis.In laboratories equipped with Fluorescent Microscope FLUOROCHROME Staining is more sensitive than ACID FAST staining for identification of Tubercle bacilli.
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